Glass Based Arrays (G-Series)
Glass Slide Antibody Arrays
Semi-Quantitative, Sandwich-based, Glass Slide Antibody Arrays
RayBio® Glass Slide-based Antibody Arrays (G-Series) were introduced as a “second generation” array platform after the chemiluminescent membrane arrays (C-Series). The G-Series uses a 75mm x 25mm glass chip platform, which delivers easier handling along with the use of much lower sample volumes. The glass slides also utilize a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence.
G-Series Antibody Arrays are particularly well-suited for projects with very limited sample volumes (as little as 10 μl per array) and large numbers of samples. Dozens of arrays can be processed per day by a single technician. Furthermore, the slide chamber assembly is compatible with many automated array processing systems, allowing high-throughput studies to be performed with very little hands-on time.
- High-throughput profiling of cytokine expression
- Identifying potential molecular targets for drug development
- Identifying the molecular mechanisms of drug action<
- Identifying crucial factors involved in disease processes
- Discovering biomarkers for disease management
- Discovering expression patterns for molecular classification of diseases
- High sensitivity (pg/mL to ng/mL levels)
- Large dynamic range of detection (4 orders of magnitude)
- Compatible with many sample types
- High throughput
- Low sample consumption: as little as 10 µl of original sample required per array
- No dedicated equipment required; Array scanning and Analyses service available
- Customizable – create a custom array from our list of targets
How It Works
Comparison to ELISA
- More Data, Less Sample: G-Series Antibody arrays provide high-content screening using less sample volume than ELISA. Only 50 µl needed (after dilution) to detect up to 40 different biomarker proteins. As little as 10 µl original sample!
- Global View of Cytokine Expression: Antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.
- Greater Sensitivity: As little as 4 pg/ml of MCP-1 can be detected using the G-Series array format. In contrast, the similar MCP-1 ELISA assay has a sensitivity of 40 pg/ml of MCP-1.
- Increased Range of Detection: ELISA assays typically detect a concentration range of 100- to 1000-fold, however, RayBiotech arrays can detect IL-2 at concentrations of 25 to 250,000 pg/ml, a range of 10,000-fold.
- Better Precision: As determined by densitometry, the inter-array Coefficient of Variation (CV) of spot signal intensities is 5-10%, comparing favorably with ELISA testing (CV = 10-15%).