Glycobiology Arrays

Glycobiology Arrays

A versatile and innovative series of products to detect glycosylation

Glycosylation plays an important role in a wide variety of disease processes including tumor cell metastasis, inflammation and bacterial pathogenesis. The RayBio Glycobiology Arrays are available in three different formats for detection of glycans or glycan-protein interactions. All 3 formats feature glass slide supports with fluorescent detection.

Glycan Arrays

The glycans featured in the RayBio Glycan Arrays are the most frequently identified structures showing important binding function in literature. The glycans are spotted on the array, capturing glycan-binding proteins, for example the influenza virus, which binds to a variety of sialosides with a serotype-specific pattern. Another example are Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression and function by binding beta-galactosides. The RayBiotech Glycan Arrays can be used with either a label-based method or with a sandwich-based method.

Glycome Arrays

The RayBio Glycome Arrays allow researchers to efficiently obtain glycosylation profiles of several proteins in their samples. Capture antibodies which bind known glycosylated proteins are printed onto glass slides. After incubation with the samples, five unique biotin-labeled lectins (see below) are incubated with the array to allow binding to the glycans from the captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.

Lectin Arrays

For the RayBiotech lectin array, each well of a standard glass slide is spotted with 40 different lectins. The slides come with an 8-well removable gasket which allows for the process of 8 samples using one slide. Each lectin, together with the positive controls, is arrayed in duplicate. The lectin array provides a powerful new tool for glycosylation determination, drug discovery and biomarker development; all with limited samples volumes required. The RayBiotech Lectin Arrays can be used with either a label based method or with a sandwich based method.