Cell Adhesion, Migration and Invasion

Cell Adhesion, Migration & Invasion Assays

No manual cell counting, fully quantitative data

Cell Adhesion Assays

Cell adhesion is a complex mechanism involved in a variety of processes including cell migration & invasion, embryogenesis, wound healing and tissue remodeling.

The CytoSelect Cell Adhesion Assays developed by Cell Biolabs quantify cell adhesion using a microplate reader, no manual cell counting is necessary.

CytoSelect ECM Cell Adhesion Assays quantify the adhesion of cells to one of a variety of extracellular matrix proteins. Plates are precoated with a uniform substrate layer of a single ECM protein: Collagen I, Collagen IV, Fibrinogen, Fibronectin, or Laminin. Adherent cells are quantified using either colorimetric or fluorometric detection. 
If you are studying more than one ECM protein, try the ECM Array. This kit includes a plate containing one row of each of the 5 substrates.

CytoSelect Endothelium & Epithelium Adhesion Assays measure the interactions of either leukocytes or tumor cells with vascular endothelium and epithelium, respectively. Quantitation is performed using a fluorescent plate reader.

Anoikis (Anchorage-Dependent Cell Death) Assays allow you to quantify and monitor anoikis, i.e. apoptosis resulting from the loss of adhesion to the ECM, in cells using a precoated plate. 


Cell Migration, Invasion & Wound Healing Assays

Cell migration is a highly integrated, multi-step process that plays an important role in the progression of various diseases including cancer, atherosclerosis and arthritis. There are various types and definitions of cell migration.

Cell invasion is related to, and encompasses, cell migration, except that cells do more than migrate. Invasive cells move through the extracellular matrix into neighboring tissues in a process that involves ECM degradation and proteolysis.

We offer cell migration assays in two formats:

Boyden Chamber Assays consist of a cell culture insert nested in the well of cell culture plate. Cells are seeded into the insert and migrate through the pores of the membrane at the bottom of the insert.
Gap Closure Assays create a defined area across which cells migrate. Cell migration can be monitored in real time by microscopy. These assays include our new proprietary Radius™ technology which uses a biocompatible hydrogel to create a circular area across which cells can migrate, and our Wound Healing Assay which is a more consistent alternative to the traditional scratch assay.