Cell Proliferation, Viability and Cytotoxicity

Cell Proliferation, Viability & Cytotoxicity Assays

Cell Health Monitoring

Cell proliferation rates or viability levels are good indicators of cell health. Proliferation or viability analysis is crucial for cell growth and differentiation studies, and is often coupled with metabolism analysis. Metabolic activity is commonly used as a viability indicator, but for some applications it can be important to assess viability independent of metabolic state.

Cell proliferation is also a convenient measure of population dynamics when studying cytokines or growth factors, or in bioprocess optimization work. On the other hand, assessing compound cytotoxicity is a critical step in pharmaceutical development. In oncological settings these assays  are also used to evaluate both compound toxicity and inhibition of tumor cell growth during drug development.

Cell viability in a particular experiment can depend on diverse criteria ranging from redox potential of the cell population, the ATP/ADP levels, the integrity of cell membranes, to the activity of cellular enzymes such as LDH and esterases. Therefore, biomarkers that can be quantified include ATP, NADH, caspases, LDH, and live- and dead-cell proteases.

BioCat offers a large variety of assays to measure cellular proliferation, cell viability, and cytotoxicity for monitoring the response and health of cells in culture after treatment with various stimuli.

Principle of the LDH-Cytotoxicity Colorimetric Assay Kit II

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity studies.

 

Ldh Cytotoxicity Ii

The LDH-Cytotoxicity Colorimetric Assay Kit II utilizes the enhanced WST reagent for a fast and more sensitive detection of LDH released from damaged cells. LDH oxidizes lactate to generate NADH, which then reacts with WST to generate yellow color. The intensity of the generated color correlates directly with the number of cells lysed.