myTags FISH Probes

myTags FISH Probes for Cytogenetic Applications

myTags® are oligonucleotide probes for detection and visualization of complementary genomic targets in FISH and other applications.

Each myTags® library contains unique short oligonucleotide sequences used as molecular probes to detect the presence of genomic targets and locate their spatial and temporal position in cytogenetics research, such as RNA FISH probes. The short length of myTags molecular probes, 43-47nt, allows for quick and efficient penetration of cell barriers and a consistent melting temperature range enables using a single hybridization profile for fluorescent in situ hybridization (FISH) assays.

  • Custom design process eliminates repetitive regions and boosts signal
  • Probes provided as unlabeled immortal libraries or ready-to-use labeled probes
  • Experienced in-house design team of Daicel Arbor can identify probes for any FISH project

The proprietary bioinformatics design process at Daicel Arbor Biosciences uses custom genomic coordinates from assembled sequences for identification of hundreds to thousands of highly-specific molecular probe sequences for hybridization only to the intended targets. Unlike BAC probes, myTags probes collectively provide bright and reliable signal in DNA-FISH assays while minimizing or eliminating background signal associated with repetitive sequences and non-specific binding. A proprietary oligo synthesis technology is utilized to enable parallel synthesis of tens of thousands of different probe sequences in a single pool.

Free assistance can be offered in designing probes and customer-designed probes can be incorporated into the myTags labeling system, just get in touch with us.

myTags FISH probes are successfully used in specialized cytogenetic applications such as:

  • Cryo-FISH
  • Chromosome painting for a variety of cell types.

SARS-CoV-2 Detection with myTags Expert FISH Probes

Besides the custom designed labeled and immortal probe sets Daicel Arbor Biosciences developed the premade myTags Expert SARS-CoV-2 probes. They were designed using the most recent genome available. These probe sets are specific to the SARS-CoV-2 virus and will not hybridize significantly to any other circulating human coronaviruses.

myTags FISH probes are provided as labeled, ready-to-use fluorescent probes or as immortal probe libraries to be amplified and labeled as needed using the myTags labeling protocol. Labeled probes are provided at minimum yields of 700 pmol and are available in a number of options, including fluorophores and haptens. SARS-CoV-2 probes are provided at minimum yields of 100 pmol.

you need any help?

Please contact:

Maria Weber

Tel. +49 (0) 6221 71415 16