Fluorescent Proteins

Fluorescent Proteins

Live Cell Imaging

A broad collection of bright fluorescent proteins (FPs) developed by Evrogen for a wide range of applications in the field of live-cell assays is offered. Fluorescent proteins can be used for in vivo protein localization and interaction studies, analysis of promoter activity in live cells, tracking subcellular organelles, identification and isolation of specific populations of cells, generation of stably transfected cell lines, and more. 
Ranging in color from blue to far-red, the fluorescent proteins allow for the visualization of multiple events simultaneously by both fluorescent microscopy and flow cytometry. All fluorescent proteins are improved by mutagenesis and codon usage optimization for high expression levels in mammalian cells and fast maturation at 37°C. 
They have been used successfully in prokaryotes, yeasts, animals and plants. The proteins possess bright stable fluorescence and enable monitoring of target cells or proteins over an extended period of time. No addition of cofactors or substrates is required for fluorescent protein detection. Specialized fluorescent protein-based tools like photoactivatable fluorescent proteins, fluorescent photosensitizers and biosensors are also provided, see links below. 

The Evrogen Spectra Viewer can be used to compare the spectra of the different fluorescent proteins and to calculate their excitation and emission efficiencies at different wavelengths.

Antibleaching Media as well as Antibodies for the immunological detection of fluorescent proteins are also available.

Special Offer

When ordering 2 or more vectors encoding the same fluorescent protein, you receive a 50% discount on the second and subsequent vectors.

Please use promotion code EV13 when placing your order.

 

Turbogfp

TurboGFP expression in stably transfected mammalian cell lines.
(A) Human cervix carcinoma HeLa cells with TurboGFP in cytoplasm; (B) C2C12 mouse myoblasts with TurboGFP in cytoplasm; (C) PC-12 rat phaeochromocytoma cells with TurboGFP in cytoplasm; (D) PC-12 rat phaeochromocytoma cells with TurboGFP in cytoplasm after the addition of nerve growth factor (cells differentiate irreversibly into neuron-like cells); (E) Chinese Hamster Ovary Cells CHO-K1 with TurboGFP in cytoplasm; (F) HeLa cells expressing TurboGFP-fibrillarin fusion; (G) Walker 256 rat tumor cells with TurboGFP in cytoplasm; (H) M3-mouse melanoma with TurboGFP in cytoplasm; (I) 3T3 mouse fibroblast with TurboGFP in cytoplasm; (J) T24 human bladder carcinoma with TurboGFP in cytoplasm; (K) HeLa cells expressing mitochondria-targeted TurboGFP; (L) T24 human bladder carcinoma expressing mitochondria-targeted TurboGFP.

you need any help?

Please contact:

Dr. Bettina Buchmann

Tel. +49 (0) 6221 71415 16 info@biocat.com