Stem Cell Research

Tools for Stem Cell Research

Induce and Monitor Pluripotency or Track Differentiation

Stem cells are undifferentiated cells that can remain with the capacity to develop into specialized cell types. Specific triggers are needed for a stem cell to develop into a skin, heart, or lung cell, or any of the other cell types with specific functions in the body; otherwise the stem cell maintains itself in a state of long-term self renewal via mitotic division. Due to these unique properties of differentiation and rejuvenation, stem cells are being evaluated for use in cell-based therapies for diseases such as cancer, and neurodegenerative and muscular diseases.

Pressing questions in this rapidly evolving field concern the internal and external signals for cell differentiation and the specificity of such signals for differentiation into particular cell types. While individual transcription factors and stem cell markers such as OCT4 have been identified, the revolution in stem cell research is identifying hundreds of other genes that either play a critical role in stem cell biology, or are uniquely impacted within the stem cell environment.

 

Stem Cell

 

In addition to a large selection of stem cells, induced pluripotent stem cells and feeder cells, BioCat offers comprehensive tools for investigating stem cell differentiation and pluripotency. The Stem Cell Differentiation Reporter and Pluripotency Promoter Reporter Systems apply cell-specific promoters and a fluorescent reporter for monitoring differentiation and pluripotency, respectively, in real-time. With the Pluripotency Response Reporter System you can determine the pluripotent state of stem cells using constructs expressing copGFP and luciferase in response to Oct4 or Sox2 activity. mRNA transcripts or lentiviral particles for the expression of pluripotency factors like c-myc, Klf4, Lin28, Nanog, Oct4 (POU5F1, Oct3/4), Sox2 enable cellular reprogramming. Kits for stem cell characterization using the stem cell marker alkaline phosphatase (AP) and for stem cell quantification and isolation are also available.

The CelVivo ClinoStar System creates a cell culture environment which promotes the growth, maintenance and functionality of large 3D tissue mimetic structures, whether they are spheroids, organoids or other aggregates.