Human ORFeome Clone Collections

MGC Full-ORF Human cDNA Clones in Gateway Entry Vectors

The MGC premier Human ORF Collections provide a comprehensive resource of fully annotated, sequence-verified entry ORFs derived from collections generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (in collaboration with the Broad Institute) and the international ORFeome Collaboration (OC).
The ORF clones are provided in Gateway-adapted entry vectors for fast and convenient transfer to any compatible expression vector and offered in multiple configurations to suit a variety of research needs.

• Gateway entry vectors – easily transfer to any compatible expression vector
• Available with and without stop codons – multiple options for native protein expression or the addition of C- or N- terminus peptide tags
• Sequence-verified – increased confidence in your results

Gateway Cloning Technology

Gateway cloning is a recombination-based method for quickly and efficiently creating vectors expressing tagged or native open reading frames (ORFs).

MGC premier Human ORFeome v8.1 Collection

The MGC premier Human ORFeome v8.1 Collection is the newest version of the human ORFeome developed by the Center for Cancer Systems Biology (CCSB) and represents the most fully sequenced, flexible and annotated version of the human ORFeome to date. This collection is derived from the Mammalian Gene Collection (MGC) and contains 12,071 clonally-derived ORFs that represent 11,016 human genes.
Each ORF clone is derived from a single bacterial colony and has been verified by next generation sequencing. The clones are provided in Gateway-adapted entry vector for fast and convenient transfer to any compatible expression vector. The entire collection of entry clones is available for research use without any restriction through the ORFeome Collaboration.

This collection was also subcloned into a lentiviral expression vector. The resulting MGC premier Human Lentiviral ORF Clone Collection (CCSB) is also available, please contact us.

MGC premier ORFeome Collaboration Collection

The MGC premier Human ORFeome Collaboration Collection currently represents 9,804 genes with 16,581 clones. The majority of the ORFeome collaboration targets were generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (DFCI-CCSB). These full-length, annotated and sequence verified ORFs originate from the existing collection of MGC full-ORF human cDNA clones and have been transferred into Gateway Entry vectors as a ready to use resource for recombinant protein expression. For additional convenience and versatility the ORF clones are available in two formats, with and without stop codons. The ORF clones without stop codons facilitate the synthesis of either C- or N- terminal fusion proteins and clones with stop codons enable the synthesis of native proteins in addition to N-terminal fusion proteins.

MGC premier Human ORF Complete Collection

The MGC premier Human ORF Complete Collection is our most extensive entry ORF library and includes both the ORFeome v8.1 and ORFeome Collaboration Collections. For descriptions see above.
This collection´s non-overlapping content represents 14,918 unique genes with 22,057 unique clones.