CopyRight Cloning Kits

CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC)

High efficiency cloning and library construction for your largest inserts

  • Efficiently clone any size insert up to 200 kb, with no background
  • Stabilize toxic or difficult inserts with CloneSmart® technology
  • Clone gene clusters or whole operons
  • Create libraries from A/T- or G/C-rich genomes
  • Induce BAC copy numbers for higher-yield DNA preps

Complete your large-insert BAC cloning and library construction projects with unprecedented accuracy, reliability and efficiency using CopyRight® v2.0 BAC cloning kits with pSMART® BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells. 

CopyRight kits are specifically designed for optimal cloning efficiency and versatility.  Minimize cloning bias and clone otherwise toxic genes with the pSMART BAC vector, designed with CloneSmart® transcription-free technology to stabilize difficult inserts.  The lacZ-sacB stuffer fragment eliminates uncut vector from recombinants resulting in zero background, and inducible copy number amplification (20-50X) allows high DNA yields.

Clone a single target, or generate a complete library (Figure 2) with confidence.  The CloneSmart design maintains "unclonable" inserts, ensuring sequences are not lost or rearranged in the cloning process. 

Performance Data

Copyright Arabidopsisbac 500

Figure 1. Arabidopsis Random Shear BAC Library in the pSMART BAC vector. 7,680 clones were obtained with an average insert size of 105 kb and ~5X genome coverage.

 

you need any help?

Please contact:

Dr. Monika Haas

Tel. +49 (0) 6221 71415 16 info@biocat.com