Electroporation and Chemically Competent Cells
Competent Cells for Any Application
Choosing the right cells for your cloning and protein expression applications will directly impact the time and effort required for your project as well as its success. The competent cells developed by our partners Lucigen, Genlantis, Antibody Design Labs and Codex DNA offer the best performance, value and convenience for cloning and expression.
Chemically Competent Cells offer an unmatched value for routine cloning, and Electrocompetent Cells provide the highest possible efficiency for difficult cloning and library construction. In addition, a variety of competent cells for protein expression with unsurpassed performance is available.
Please ask for bulk quantities and custom dispensing/packaging options.
- General Cloning & Library Construction
- Large or Difficult Fragment Cloning
- Phage Display Library Applications
- Protein Expression
Protein Expression with Ultra-High Yields
Vmax™ X2 cells are a next-generation bacterial expression system engineered from the gram-negative, non-pathogenic marine bacterium, Vibrio natriegens, which is amoung the fastest growing organisms on earth. Vmax™ X2 cells retain all the benefits of traditional bacterial expression systems while generating significantly greater amounts of protein.
• Exceptional growth rates, with a doubling time less than 14 minutes (up to 2x faster than E. coli)
• Protein yields are 2–4x higher than E. coli due to high biomass production and growth rates
• Protein expression can be induced at a wide variety of OD600readings (0.1 to over 1.0)
• Compatible with commonly used plasmids and antibiotics
• 100x reduction in endotoxin levels compared to traditional coli strains
Features of Lucigen Competent Cells
• Clone unstable sequences with reliable results
• Cells of choice for lentiviral cloning and CRISPR/Cas9 genome editing
• Direct replacement for Stbl3 and SURE strains
• High efficiency: > 1 ×108 cfu/ug (chem) or > 1 × 1010 cfu/ug (electro)
Phage Display Cells
• TG1, SS320 and E2738 strains
• Perfect for antibody or peptide phage display libraries
• Highest transformation efficiencies available anywhere
• Amber suppressor and non-amber suppressor lines for maximum flexibility
• ClearColi BL21(DE3) and K-12 strains
• E. coli plasmid and protein production strains lacking endotoxic LPS
• Ideal for mammalian cell transfection, immunogenicity and toxicity assays
• Reduce false positives in cytokine assays, improve transfection results
• OverExpress C41(DE3), C43(DE3), C41(DE3)pLysS and C43(DE3)pLysS strains
• Effective in expressing toxic and membrane proteins from any organism
• Cited in hundreds of publications
• Combo pack containing all four strains available
E. cloni 10G and 10GF’ Cells
• Your most cost-effective source for competent cells
• Transformation efficiencies up to > 4 × 1010 cfu/ug
• Broad line of strains for all your cloning needs
• Direct replacement of TOP10, DH5alpha, DH10B, JM109, XL1-Blue strains
Please contact us for more information related to custom competent cell services or to receive a bulk quotation.