Histone Modification Assays

Histone Modification Assays

Screen and measure up to 21 different H3 or 10 different H4 modifications in ELISA-like kits

Histones are primary protein components of eukaryotic chromatin and play a role in gene regulation. H3 and H4 histones have tails protruding from the nucleosome that can be modified post-translationally to alter the histone's interactions with DNA and nuclear proteins, leading to epigenetic changes for regulating many normal and disease-related processes. A complete series of kits for the quantification of methylation, acetylation, and phosphorylation of H3 histones at all sites is offered. The most comprehensive selection of Histone Modification research products covers every step of your experimental workflow, from upstream to downstream.

In addition, EpiQuik Histone Modification Multiplex Assay Kits for measuring multiple histone H3 or histone 4 modifications simultaneously are provided.

Histone Methylation

Histone methylation causes transcription repression or activation, depending on the target sites. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. Measurement of histone methyltrasferase activity and quantification of histone methylation patterns have become pivotal in studying epigenetic regulation of genes, as well as inhibitor discovery.

Histone Acetylation

Histone acetylation and histone deacetylation involve the addition or removal of an acetyl group on lysine residues in the N-terminal tail and on the surface of the nucelosome core of histone proteins. Acetylated and deacetylated histones are considered epigenetic tags within chromatin by relaxing (euchromatin) or tightening (heterochromatin) chromatin structure, subsequently increasing or decreasing gene transcription levels.

Histone Phosphorylation, Sumoylation and Citrullination

Various histone modifications, such as histone citrullination and histone phosphorylation, have been shown to epigenetically impact gene expression through different mechanisms. Adjusting epigenetic marks and changing chromatin structure – known as chromatin remodeling – as a result of these histone modifications can influence gene regulation in many tissue types. Studying these histone modifications provides researchers with valuable insight into cellular processes such as apoptosis, cell growth, stress response, and transcriptional regulation.

you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com