Recombinant Nucleosomes (rNucs)

Nucleosomes: Nature’s preferred chromatin substrate

Recombinant Nucleosomes (rNucs) are human recombinant nucleosomes comprised of histone proteins made in E. coli wrapped with fully synthetic Widom 601 DNA. As the nucleosome is the true physiologic substrate for many histone modifying enzyme, these are excellent substrates for enzyme screening assays.

EpiCypher has an extensive library of recombinant nucleosomes, including

• rNucs wrapped with hemi-methylated and methylated DNA templates
• Unmodified rNucs, which can be used as controls in chromatin assays
• rNucs assembled with histone variants (i.e. H2AZ)