Exosome Depletion

Efficient depletion of exosomes from serum

Most culture media used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of cow exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.

• Efficient depletion of cow exosomes from fetal bovine serum or any other serum source
• Deplete exosome-sized vesicles from versatile serum volumes of up to 280 mL
• No protease treatment required
• No time-consuming ultracentrifugation
• No precipitation reagents or overnight incubation required
• Depleted FBS has no detectable cow microRNAs
• The depleted FBS provides the same cellular growth rates as the standard FBS
• Purification is based on Norgen’s proprietary silicon carbide resin separation matrix