CRISPR sgRNA Cloning & Control Vectors

The CRISPR/Cas9 system can be used for knocking out gene expression in vivo or in vitro by using the combination of a sgRNA (single guide RNA) along with Cas9 (dCas9) nuclease. Achieve permanent 100% knockout in your cell line by using Cellecta´s lentivirus-based CRISPR system. Expression of the sgRNA and Cas9 are stable and can be used in dividing or non-dividing cells or whole model organisms.
A comprehensive collection of linearized, ready-to-use sgRNA cloning vectors developed by Cellecta and OriGene as well as different control vectors (non-targeting, sgCopGFP, sgPCNA, sgPOLR2L) is offered.

Two Vector CRISPR/Cas9 System

The Two Vector CRISPR/Cas9 System is used to perform functional knockout screens in vivo or in vitro by expressing sgRNA (single guide RNA) and Cas9 nuclease from different lentiviral vectors. It allows for faster knockout of the target gene in cells by first selecting cells expressing high levels of Cas9. This system is recommended when using complex sgRNA libraries in screening approaches.

• Pre-transduce with Cas9 lentivirus to decrease noise in your knockout screen
• Make high-titer sgRNA lentivirus for efficient transduction
• Vectors contain different antibiotic resistance genes to ensure stability and easy selection
• Tet-inducible and constitutive U6 expression available

Single Vector CRISPR/Cas9 System

The Single Vector CRISPR/Cas9 System comprises all-in-one constructs expressing  sgRNA and Cas9 nuclease from the same vector.

• Co-transduction not necessary since sgRNA and Cas9 nuclease are expressed from one vector
• Lentiviral vectors integrate into the host cell genome and are passed on to daughter cells
• Cells can be treated, grown for several passages, frozen and thawed - the lentiviral construct remains in the host cells
• Vector contains antibiotic selection to ensure stability