X-CLARITY Tissue Clearing System

Tissue Clearing for High-resolution 3D Imaging

Clearing – Labeling – Imaging

X-CLARITY™ is based on the CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging / Immunostaining /in situ-hybridization-compatible Tissue hydrogel) method. With CLARITY, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream.

 

X Clarity 4 Steps Tissue Clearing

Key Features of the X-CLARITY Tissue Clearing System

  • Collection of systems and ready-to-use reagents
  • Simplified and standardized tissue clearing
  • Rapid, safe, efficient and reproducible
  • Non-destructive
  • Endogenous fluorescent protein expression kept intact
  • Adaptable for various tissues

Components of the X-CLARITY Tissue Clearing System

The CLARITY  Tissue Clearing system represents a collection of ready-to-use reagents and dedicated instrumentation. It clears a variety of tissues and organs in a simple and standardized manner.

Every component plays a specific role in the clearing process.

X Clarity Tcs Ii 02 Klein

Hydrogel Infusion
A fixed tissue sample is incubated in the X-CLARITY™ Hydrogel Solution Kit to allow hydrogel monomers to diffuse uniformly throughout the sample.

X-CLARITY™ Polymerization System
Monomers polymerize in the anaerobic environment, linking the biomolecules to a hydrogel network, which preserves molecular information and structural integrity. This step is facilitated by the use of the X-CLARITY™ Polymerization System.

X-CLARITY™ Tissue Clearing System II
Once the tissue-hydrogel hybrid has been formed, the hybrid is cleared in the X-CLARITY™ Tissue Clearing System II using the ready-to-use Electrophoretic Tissue Clearing Solution. Lipids are extracted actively through electrophoresis or passively, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping.

Performance

Whole Thy1-YFP Mouse Brain

Whole Thy1 Yfp Mouse Brain

The Thy1-YFP signal is preserved and vibrant after tissue clearing with the X-CLARITY™ systems and reagents. Courtesy of Hyung Jin Choi, PhD Seoul National University College of Medicine.

 

Mouse Embryo

Mouse Embryo
Mouse embryo (E 21.5) cleared with the X-CLARITY™ systems and reagents. Courtesy of Michal Shoshkes-Carmel, PhD and Andrea Stout, PhD, University of Pennsylvania.