Tissue Clearing for High-resolution 3D Imaging

Clearing – Labeling – Imaging

X-CLARITY™ is based on the CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging / Immunostaining /in situ-hybridization-compatible Tissue hydrogel) method. With CLARITY, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging.

Key features of the X-CLARITY Tissue Clearing System

• Collection of systems and ready-to-use reagents
• Simplify and standardize tissue clearing
• Rapid, safe, efficient and reproducible
• Non-destructive
• Keeps endogenous fluorescent protein expression intact
• Adaptable for various tissues

Components of the X-CLARITY Tissue Clearing System

The CLARITY  Tissue Clearing system represents a collection of ready-to-use reagents and dedicated instrumentation. It clears a variety of tissues and organs in a simple and standardized manner.

Every component plays a specific role in the clearing process.

Hydrogel Infusion:
A fixed tissue sample is incubated in the X-CLARITY™ Hydrogel Solution Kit to allow hydrogel monomers to diffuse uniformly throughout the sample.

X-CLARITY™ Polymerization System:
Monomers polymerize in the anaerobic environment, linking the biomolecules to a hydrogel network, which preserves molecular information and structural integrity. This step is facilitated by the use of the X-CLARITY™ Polymerization System.

X-CLARITY™ Tissue Clearing System II:
Once the tissue-hydrogel hybrid has been formed, the hybrid is cleared in the X-CLARITY™ Tissue Clearing System II using the ready-to-use Electrophoretic Tissue Clearing Solution. Lipids are extracted actively through electrophoresis or passively, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping.