Study the complete set of epigenetic modifications with NGS approaches
Epigenetics most often involves changes that affect gene activity and expression. The term also refers to functionally relevant changes to the genome that do not involve a change in the nucleotide sequence. Examples of mechanisms that produce such changes are DNA methylation and histone post-translational modification (PTM), each of which alters how genes are expressed. Gene expression can also be controlled through the action of repressor or activator proteins that attach to specific regions of the DNA.
Analysis of protein interactions with DNA: CUT&RUN, CUT&Tag and ChIP-seq
CUT&RUN-sequencing, also known as cleavage under targets and release using nuclease, is an innovative method to analyze protein-DNA interactions. It combines antibody-targeted controlled cleavage by micrococcal nuclease with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.
Discover EpiCypher's CUTANA CUT&RUN platform, including kits and compatible antibodies against histone PTMs and chromatin-associated protein targets that have been rigorously tested for reliable and robust performance in CUT&RUN assays.
CUT&Tag-sequencing, also known as cleavage under targets and tagmentation, is a method used to analyze protein interactions with DNA. CUT&Tag-sequencing combines antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.
Discover the innovative CUTANA CUT&Tag products from our partner EpiCypher.
ChIP-sequencing, also known as ChIP-seq, is a more tarditionl method also used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest.
Currently, ChIP-Seq is still the most common technique utilized to study protein–DNA relations, however, it suffers from a number of practical and economical limitations that CUT&RUN and CUT&Tag sequencing do overcome.
Analysis of DNA methylation: Methyl-seq
DNA methylation is the most widely studied epigenetic modification. It is often associated with the regulation of gene expression through its presence or absence in CpG Islands among gene promoter regions.
Methyl-Sequencing (also known as bisulfite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation. Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected, yielding single-nucleotide resolution information about the methylation status of a DNA fragment.
BioCat offers different Methyl-Seq DNA and RNA Library Kits to study this so-called 5th base of the genome.