CUTANA CUT&RUN Assays
CUTANA™ CUT&RUN: Go from cells to data in < 4 days
The Cleavage Under Targets and Release Using Nuclease (CUT&RUN) method builds upon Chromatin ImmunoCleavage (ChIC) technology.
In CUT&RUN, a fusion of protein A, protein G and micrococcal nuclease (pAG-Mnase) is used to selectively cleave antibody-labelled chromatin. This strategy eliminates immunoprecipitation steps, greatly simplifying the assay workflow. Clipped chromatin fragments are isolated from solution and used for NGS.
CUT&RUN offers clear advantages over ChIP:
- Reduced cell input : Compatible with as few as 5,000 cells
- Diverse target profiling : Histone PTMs and chromatin-interacting proteins (including remodelers)
- Low background: Fewer required sequencing reads per sample (3-5 million)
- Cost-effective: Reliable, robust, streamlined workflow
CUT&RUN has superior signal : noise with > 10-fold reduced seq depth compared to ChIP-seq