CUTANA CUT&Tag Assays
CUTANA™ CUT&Tag: Ultra-low input chromatin mapping
Cleavage Under Targets and Tagmentation (CUT&Tag) is a novel immunotethering technology, based on Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.
In CUT&Tag, a fusion of protein A, protein G, and Tn5 transposase is used to catalyze simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin. The use of Tn5 in CUT&Tag is a key advance, as this step eliminates the need for chromatin fragmentation as well as expensive library preparation.
CUT&Tag assays display significantly improved signal-to-noise (S/N) vs. ChIP-seq assays, particularly for mapping histone PTMs in ultra-low sample inputs.
CUTANA CUT&Tag Kits
The CUTANA CUT&Tag Kits offer a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). These kits use an exclusive Direct-to-PCR strategy to go from cells to PCR-amplified sequencing libraries in one tube, bypassing traditional library prep and minimizing sample loss. The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples and lightly cross-linked nuclei or cells.
- Cells to sequencing in 2 days
- Exclusive single-tube protocol, no library prep
- Designed for multi-channel pipetting and 8-strip tubes
- Reliable data down to 10,000 cells
- Only 5-8 million sequencing reads
- All necessary reagents included
How it works
Quality data with lower input and sequencing depth requirements
Kaya-Okur et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Comm. 10, 1930 (2019). (PMID: 31036827)
Kaya-Okur et al. Efficient low-cost chromatin profiling with CUT&Tag. Nat. Protoc. 15, 3264-3283 (2020). (PMID: 32913232)
Henikoff et al. Efficient transcription-coupled chromatin accessibility mapping in situ. by nucleosome-tethered tagmentation. Elife 16;9:e63274.(2020). (PMID: 33191916)