CUTANA CUT&Tag Assays
CUTANA™ CUT&Tag: Ultra low input chromatin mapping assays
Cleavage Under Targets and Tagmentation (CUT&Tag) is a novel immunotethering assay, based on Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.
In CUT&Tag, a fusion of protein A, protein G, and Tn5 transposase is used to catalyze simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin. The use of Tn5 in CUT&Tag is a key advance, as this step eliminates the need for chromatin fragmentation as well as expensive library preparation.
CUT&Tag assays display significantly improved signal-to-noise (S/N) vs. ChIP-seq assays, particularly for mapping histone PTMs in ultra-low sample inputs.
Quality data with lower input and sequencing depth requirements
Kaya-Okur et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Comm. 10, 1930 (2019). (PMID: 31036827)
Kaya-Okur et al. Efficient low-cost chromatin profiling with CUT&Tag. Nat. Protoc. 15, 3264-3283 (2020). (PMID: 32913232)
Henikoff et al. Efficient transcription-coupled chromatin accessibility mapping in situ. by nucleosome-tethered tagmentation. Elife 16;9:e63274.(2020). (PMID: 33191916)