CUTANA CUT&RUN Assays

CUTANA™ CUT&RUN: Go from cells to data in < 4 days

The Cleavage Under Targets and Release Using Nuclease (CUT&RUN) method builds upon Chromatin ImmunoCleavage (ChIC) technology.

In CUT&RUN, a fusion of protein A, protein G and micrococcal nuclease (pAG-Mnase) is used to selectively cleave antibody-labelled chromatin. This strategy eliminates immunoprecipitation steps, greatly simplifying the assay workflow. Clipped chromatin fragments are isolated from solution and used for NGS.

 

Cutana Cur Subcat Worflow Neu

 

CUT&RUN offers clear advantages over ChIP:

  • Reduced cell input : Compatible with as few as 5,000 cells
  • Diverse target profiling : Histone PTMs and chromatin-interacting proteins (including remodelers)
  • Low background: Fewer required sequencing reads per sample (3-5 million)
  • Cost-effective: Reliable, robust, streamlined workflow

CUT&RUN has superior signal : noise with > 10-fold reduced seq depth compared to ChIP-seq

Cutana Sperior Signals

A representative 350 kb region of H3K4me1 profiles in K562 cells, generated using CUT&RUN (yellow tracks), native ChIP-seq (blue tracks), or cross-linked ChIP-seq (green tracks). All data were generated by EpiCypher and are expressed as reads per million (RPM). Color-coded gradient (to right) represents signal-to-noise ratios determined by genome-wide analysis (bamFingerprint data, not shown).

you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com