Targeted Sequencing

Hybridization Capture Kits using biotinylated RNA Baits

myBaits® target capture kits can greatly enhance the efficiency and cost-effectiveness of NGS research projects for any organism and any project size

The Kits from Daicel Arbor Biosciences provide pools of in-solution biotinylated RNA probes plus reagents for highly-efficient, scalable targeted sequencing on any NGS platform, including Illumina®, Ion Torrent®, PacBio®, and Nanopore®. Daicel Arbor Biosciences’ proprietary oligo synthesis technology delivers high-quality, complex baits at extremely competitive pricing. In addition, we can offer complimentary bait design and project development assistance from a team of expert scientists.

myBaits kits have been successfully used in thousands of research projects from a wide variety of genome types (e.g. animals, plants, and microbes) and DNA sources (e.g. fresh, degraded, and environmental). 

myBaits Custom Kits

Kits tailored for specific target regions are the most popular choice, allowing enrichment of specific SNPs, exons, genes, and other sequence motifs from genomic or metagenomic samples.

  • Target Capture Kits for DNA- and RNA-Seq
  • Target Capture Kits for Methyl-Seq

myBaits Expert Kits with Predesigned Panels

Daicel Arbor Biosciences also offers a variety of predesigned kit options for specific research applications, such as mitochondrial DNA sequencing, ultraconserved element sequencing, whole-genome enrichment from metagenomic samples, and human cancer exome research.

Latest addition to the targeted sequencing portfolio is a kit for human population genomics research.

  • Human Affinities
  • Virus - SARS-CoV-2
  • 16S-Hyb
  • Wheat Genome
  • Mito Panel
  • Onconome
  • UCE (Ultraconserved Elements)
  • Plant - Angiosperms
  • Plant - Compositae
  • Whole Genome Enrichment (WGE)

How does myBaits work?

 

Mybaits Minioverview Horizontal V2

Figure 1.
(1) HYBRIDIZATION
– An NGS library is denatured via heat, and allowed to hybridize to a complex mixture of complementary biotinylated RNA baits over the course of several hours. Adapter-specific blocking oligos prevent random annealing of library molecules at the common adapter sites.
(2) WASHING – After the hybridization is complete, the biotin present on each bait is bound to a streptavidin-coated magnetic bead. Wash steps help remove off-target or poorly-hybridized library molecules.
(3) AMPLIFICATION – The remaining library molecules that are still bound to their complementary baits are denatured via heat, and amplified using universal library primers. This “enriched” library can now be sequenced.

 

Understanding myBaits Custom Performance

Hybridization capture with myBaits Custom is an extremely versatile and flexible technology that has been applied to an enormous variety of applications and taxa, such as plants, animals, humans, bacteria, and viruses. Hundreds of studies have been published with myBaits over the years. Across the wide variety of applications, myBaits Custom kits frequently achieve high on-target read percentages and/or high unique read complexity for most applications, creating many orders of magnitude savings in sequencing compared to shotgun approaches. In addition, research scientists are continually innovating novel improvements to the speed, ease of use, and performance of our myBaits kits across a variety of applications. We can always provide specialized experimental design advice to maximize success with your next NGS targeted sequencing project.

Typical example of visualized NGS reads

 

Mybaits Ngs Reads

Figure 2. NGS reads from enriched human gDNA library. Single-end NGS reads are shown aligned to the hg38 genomic reference sequence. Positions of the four 80nt myBaits probes in this region are indicated by blue bars. Unique read coverage is highest across the baited region, and tapers off upstream and downstream of the baited region. If sequencing further into the known or unknown flanking regions is desired, simply increase the length of your NGS library molecules (compatible with any myBaits kit).

you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com
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