Swift Amplicon Panels

Swift Amplicon NGS Panels

Overlapping Amplicon Coverage in a Single Tube

The Swift Amplicon NGS Panels are available as expertly "Pre-Designed NGS Panels"  using content from peer-reviewed publications and thought leader input. These Panels include assays for cancer genes, rare disease and sample tracking.

However if you want to to start from scratch and generate a unique panel covering exactly what you need the "Custom NGS Panels" are the right choice for you. Our experts will help you through the design process and validation of your specific panel. 

All Swift Amplicon NGS Panels are offered in two formats

Accel-Amplicon® NGS Panels utilize multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries.

Swift Normalase® Amplicon Panels (SNAP) utilize a high throughput compatible  workflow readily compatible with the downstream enzymatic Swift library normalization technology. They are also based on multiple overlaping amplicons in a single tube and are using the same rapid  2-hour workflow. 

Accel-Amplicon® NGS Panels

Prepare Targeted Libraries in 2 Hours

Accel-Amplicon NGS Panels utilize multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries. Primer pairs in Accel-Amplicon NGS Panels are compatible with short DNA fragments and are designed for generating libraries from input DNA as low as 10 ng. This unique design enables applications utilizing limiting or damaged samples such as FFPE and cfDNA, and provides powerful solutions for detecting variants and screening clinically-relevant mutations with limit of detection down to 1%.

Key Benefits:

  • Single-tube assay - provides easy and consistent processing
  • Ready-to-sequence libraries in 2 hours
  • As low as 10 ng input DNA required - process low input samples
  • Amplicon sizes of 140 bp average - compatible with FFPE and cfDNA
  • Contiguous coverage - complete coverage of desired regions
  • Designed for germline and somatic variant detection
  • Provides high on-target percentage (> 95%) and coverage uniformity (> 95%), enabling low frequency (> 1%) variant discovery and confirmation

Swift Normalase® Amplicon Panels (SNAP)

Target and Normalize in 2.5 Hours

Swift Normalase Amplicon NGS Panels (SNAP) utilize multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries. The PCR1+PCR2 workflow generates robust libraries even from low input quantities of DNA that may be subsequently quantified and normalized with conventional methods such as Qubit® or Agilent Bioanalyzer, or optionally using the included Swift Normalase reagents.

Primer pairs used in SNAP are compatible with short DNA fragments and are designed for generating libraries from human input DNA as low as 10 ng or pathogen RNA/DNA in the picogram range. This unique design enables applications utilizing limiting or damaged samples such as FFPE and cfDNA, and provides powerful solutions for detecting variants and screening clinically-relevant mutations with limit of detection down to 1%.

Key Benefits:

  • Integration of the revolutionary Swift Library Normalization Technology
  • Multiplex hundreds to thousands of targets in a single tube PCR
  • Robust yields from low input samples using PCR1+PCR2 workflow
  • Supports high throughput library quantification methods (fluorometric, electrophoretic, or Swift Normalase)
  • Designed for germline and somatic variant detection, or targeted pathogen sequencing
  • Provides high on-target percentage and coverage uniformity
  • compatible with FFPE and cfDNA

Bioinformatics

Following sequencing, the data generated must be computationally aligned to a reference genome and variant calling performed to assess the status of genetic mutations, insertions, and deletions present in the sample. With the VarSome platform from Saphetor, users of Swift Amplicon panels can simply upload their sequencing FASTQ or VCF files into the secure cloud-based VarSome platform and leverage the rich set of features available to analyze and annotate the resulting data.

 

you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com
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