DriverMap Adaptive Immune Receptor (AIR) TCR-BCR Profiling

Profile the CDR3 repertoire of all T-cell receptor (TCR) and B-cell receptor (BCR) chains in a single-tube, single-day assay

DriverMap AIR is the only assay technology on the market that profiles the CDR3 repertoire of all T-cell receptor (TCR) - TRA, TRB, TRG and TRD - and B-cell receptor (BCR) - IGH, IGK and IKL - chains in an single-tube, single-day assay using multiplex PCR-NGS technology without the need of any additional specialized equipment.

• Start from all immune sample types such as whole blood, PBMC, cancer biopsies, tissue samples, FFPE or dried blood microsamples
• Profile directly from immune cells (e.g.,100-50,000 FACS-sorted cells) without the need to isolate RNA
• Detect and accurately quantify more AIR clonotypes with Unique Molecular Identifiers (UMIs)
• Amplify only functional TCR/BCR mRNA isoforms, without pseudogenes, ORFs or misarranged genes

How It Works

DriverMap Multiplex PCR technology uses gene-specific primers which significantly reduce the level of non-specific binding and primer-dimer amplification products and are designed to target only expressed TCR/BCR mRNA isoforms. Unique Molecular Identifiers (UMIs) facilitate accurate quantitation of the copy number of cDNA molecules in amplification steps, as well as detection of low abundance clonotypes and correction of amplification biases and sequencing errors. Furthermore, the dual-index amplicon labeling strategy minimizes index hopping during NGS allowing for comprehensive readouts.
Full profiles of the antigen-recognition CDR3 region enable assessment of CDR3 length distribution, V(D)J segment usage, isotype composition for BCRs, somatic mutations, and similar characteristics with immune receptor profiling software such as MiXCR (MiLabs).

Applications of BCR Sequencing

• Identify broadly neutralizing antibodies (BNAbs) and map Ig-seq datasets to known antibody structures for antibody and vaccine development
• Track B-cell migration and development patterns
• Find clinical markers of autoimmune diseases such as multiple sclerosis, rheumatoid arthritis and cancers (e.g. B-cell lymphoma)
• Contrast naïve and antigenically challenged datasets for understanding antibody maturation

Applications of TCR Sequencing

• Track T-cell clonality and diversity for mechanism of action of immune checkpoint inhibitors for immunotherapies
• Assess TCR overlap between repertoires to define spatial and temporal heterogeneity of the anti-tumoral immune response
• Analyze TCR sequence and structure to annotate antigenic specificity for developing personalized cellular immunotherapies

Number of clonotypes for 7 TCR/BCR chains identified in 50ng of normal PBMC or whole blood RNA (10x10^6 reads per sample, triplicates).

DriverMap Adaptive Immune Receptor (AIR) TCR-BCR Profiling Service

Do you have immune samples you would like to profile? Provide us with some information on your samples and your study goals, and we will get back to you to discuss your project.