Dye-based qPCR

SensiFAST SYBR One-Step Kits

SensiFAST™ SYBR® One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube.

The SensiFAST SYBR® One-Step Kits use a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

  • Optimized 1-step buffer for highly-efficient reverse transcription and amplification
  • Improved consistency between technical replicates
  • Reproducible, accurate results in as little as 40 minutes
  • Antibody-mediated hot-start minimizes non-specific amplification
  • Minimized primer-dimer amplification for improved specificity and sensitivity

To find the optimal SensiFAST kit for your real-time PCR instrument and application, you can also use this
Real-Time PCR Selection Chart

you need any help?

Please contact:

Dr. Monika Haas

Tel. +49 (0) 6221 71415 16 info@biocat.com