SensiFAST SYBR One-Step Kits

SensiFAST™ SYBR® One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube.

The SensiFAST SYBR® One-Step Kits use a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

Optimized 1-step buffer for highly-efficient reverse transcription and amplification

• Improved consistency between technical replicates
• Reproducible, accurate results in as little as 40 minutes
• Antibody-mediated hot-start minimizes non-specific amplification
• Minimized primer-dimer amplification for improved specificity and sensitivity