High‑fidelity polymerases remove erroneous bases incorporated in the growing DNA strand, increasing the accuracy of DNA synthesis from template DNA. For cloning and expression of amplified product, mutagenesis studies and related applications, proofreading enzymes should be used.
All-in-one-tube mastermixes offer a high level of convenience and improve the speed and accuracy of the PCR set-up by reducing the number of pipetting steps and risk of human errors.