There is an increasing demand for high-quality, small-scale protein preparations for analytical purposes. Protein purification is crucial for researchers who work on proteomics, functional genomics, clinical studies, differential expression, protein trafficking, and protein structural and functional studies. The most commonly used protein sample preparations include but are not limited to total protein extraction from cultured cells or tissues, plasma membrane protein isolation/purification and cell fractionations.
In recent years spin column-based technology has emerged as an alternative for solution-based extraction. This next generation technology couples traditional methods with a specially designed spin column for rapid and efficient protein extraction. Total protein can be extracted from cultured cells or tissues in as little as 1 min with high protein yield and a complete protein profile without any bias. In comparison to traditional methods, spin column-based technology represents a technological breakthrough and competes favorably with traditional methods in terms of ease of use, speed and performance.
The total protein extraction kits offered use a simple spin column to eliminate non-systematic protein loss during cell or tissue lysis. This represents a significant improvement over traditional, solution-based methods such as RIPA buffer and sonication, which are notoriously variable and fail to achieve a complete protein profile. Compatible with small sample sizes from any living organism and tissue type, the kits for total protein extraction provide you with an unaltered endogenous baseline for more accurate protein quantitation and analysis.
A key tool in understanding protein trafficking is subcellular fractionation. The subcellular fractionation kits provided eliminate the need for ultracentrifugation and make the entire workflow much easier. Following simple, well-defined protocols and using a convenient spin column format, you can obtain preparations of various organelles or other subcellular fractions, including cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions in less than one hour.
Our protein analysis reagents are designed to enhance your protein and peptide research. We offer reagents for protein solubilization, endotoxin removal, protein precipitation, Western blotting, immunoassay enhancement, red blood cell lysis and more.