Custom Cloning Services
Let the experts do the work and get the constructs you need the easier way
Custom cDNA Cloning
BioCat cooperates with partners who are well experienced with cDNA cloning and custom constructs. We are able to offer cloning services for a large selection of gene delivery platforms.
Custom HR Donor Design and Cloning
An important first step in CRISPR/Cas9 technology is the careful design and construction of homologous recombination (HR) donors. Save yourself time and trouble and have experienced specialists handle this first step for you.
Custom sgRNA Cloning
Knockout, knockdown, or activation of your target gene with CRISPR, CRISPRi, or CRISPRa sgRNA lentiviral constructs. Use flexible design services for sgRNA targeting your desired genes, isoforms, or regulatory elements.
Custom sgRNA Library Cloning
Custom-designed CRISPR sgRNA Pooled Libraries targeting virtually any set of genes and incorporating guide sequences and vector features for a range of screening applications.
Custom shRNA Cloning
Within the shRNA Cloning Service a proprietary shRNA design delivers approximately 70% shRNAs that generate >70% transcript knockdown in standard cell lines as measured by qRT-PCR. Typically 3-5 constructs are designed and cloned that target your transcript.
Custom shRNA Library Cloning
Pooled lentiviral-based libraries containing heterogeneous mixtures of shRNA allow you to assay the effects of many thousands of pooled constructs in one experiment. Taking advantage of array-based oligonucleotide synthesis, precisely defined large custom pooled libraries expressing many thousands of shRNAs canbe readily made.
Custom siRNA Cloning
The siRNA Cloning Service delivers over 70% gene knockdown efficiency in appropriate target cells, with over 70% transfection efficiency for any human, mouse, or rat gene.