The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.
An advanced technology for qPCR and SNP genotyping, the BHQplus™ Probe is a compact oligo fortified with our signature duplex stabilising chemistry to elevate the melting temperature and enhance target specificity. BHQplus Probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.
A large selection of fluorophore-quencher combinations for dual-labeled probes is available. After synthesis the high quality BHQ Dual-Labeled FRET Probes (TaqMan Probes) are purified by HPLC to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.
Scorpions™ Primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release.
Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC, Biosearch Technologies currently offers Molecular Beacons labeled with either a Black Hole Quencher™ or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.