Liquid biopsy collects samples such as blood, urine, saliva and stool and is significantly less intrusive than surgical biopsy. It provides access to cell-free DNA (cfDNA), enabling early detection of cancer cells (before a tumour develops) and together with RNA detects non-shedding cancers. Liquid biopsies provide an easier and safer mode to monitoring disease recurrence, therapeutic selection and effectiveness.
Molecular diagnostic tests are progressively moving towards lyophilized and air-dried formats. Lyo-Ready and Air-Dryable qPCR master mixes are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As ready-to-use mixes, they require no further optimization besides the addition of primers and probes.
The Air-Dryable Direct DNA qPCR Plant master mix is designed for the development of ambient temperature qPCR assays for direct detection of plant DNA from crude lysates without extraction.