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Recommended Peptide Purity Levels

Peptide purity plays a critical role in the success and integrity of your research projects. Here is a list of recommended purity levels for some common peptide applications for your reference. 

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Crude or higher by HPLC

  • Non-sensitive screening
  • Sequence optimization
  • Protein-protein interactions studies
  • Receptor-ligand interactions studies 

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75% to 85% by HPLC

  • Peptide arrays
  • ELISA testing

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85% to 90% by HPLC

  • Epitope mapping
  • Phosphorylation assays
  • Coating tissue culture plates for cell attachment
  • Protein electrophoresis applications
  • Non-quantitative or semi-quantitative enzyme-substrate studies
  • Peptide antigens for affinity purification
  • Peptide antigens for antibody production
  • Non-quantitative peptide blocking studies for Western Blot
  • Coupling to chromatography resins for affinity purifications

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>95% by HPLC

  • In-vitro bioassays & studies
  • NMR studies
  • Enzymology
  • Quantitative receptor- ligand interactions studies
  • Quantitative blocking and competitive inhibition studies
  • Quantitative phosphorylation studies
  • Quantitative proteolysis studies

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>98% by HPLC

  • Sensitive Bioassay
  • Cosmetic peptides
  • Active pharmaceutical ingredients
  • Clinical trials
  • Crystallography
  • SAR studies

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More Guidelines and FAQs

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  Peptide Design Guideline

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  Peptide Handling Guideline

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  Peptide Synthesis FAQs

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