Histone H3K4me3 Antibody: SNAP-ChIP Certified, CUTANA CUT&RUN Compatible

CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

User Manual Data sheet

Product Description

Although the specificity of the Histone H3K4me3 Antibody in CUT&RUN has yet to be empirically determined in situ using spike-in controls, CUT&RUN data profuced by this antibody show a genome-wide enrichment pattern characteristic of HeK4me3 and highly correlate with ChIP-seq.


13 0041 Gwa 400

Figure 1: ChIP-seq and CUT&RUN genome wide analysis EpiCypher H3K4me3 antibody (Catalog No 13-0041) was tested in native ChIP-seq (A) and CUT&RUN (B) using the methods described above. Genome-wide analysis of H3K4me3 enrichment (signal intensity) flanking annotated transcription start sites (TSSs; +/- 3kb) is graphed as a cumulative histogram plot (top) and shown in a heatmap (bottom). Individual gene loci in each row of the heatmap are colored by signal intensity and sorted by strongest to lowest enrichment (top to bottom). EpiCypher H3K4me3 antibody displays a characteristic enrichment pattern proximal to TSSs in both ChIP-seq and CUT&RUN.


Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.

References

Grzybowski et al (2015) Mol Cell 58:886
Shah et al (2018) Mol Cell 72:162

  • Catalog Number
    13-0041-EPC
  • Supplier
    EpiCypher
  • Size
  • Shipping
    Blue Ice
  • Price
  • 670,00 €
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Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com