BRD4 Antibody: CUTANA Compatible (CUT&RUN)

CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

Data sheet

Product Description

BRD4 antibody produces CUT&RUN peaks primarily flanking transcription start sites (TSSs, Figure 1). BRD4 peaks show a large degree of overlap with BRG1/SMARCA4 peaks (Figure 2), as has been reported in the literature (Conrad et al., 2017).

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Figure 1: BRD4 enrichment at annotated TSSs in CUT&RUN. CUT&RUN was performed using 500,000 K562 cells with BRD4 antibody as well as control antibodies (IgG negative control, EpiCypher 13-0042; H3K4me3 positive control, EpiCypher 13-0041). Sequencing reads were aligned to annotated TSSs (+/- 2 kbp) of 18,793 genes. High, medium, and low signal is ranked by intensity (top to bottom) and reflected by red, yellow, and blue colors, respectively. All rows aligned relative to H3K4me3 antibody.

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Figure 2: BRD4 CUT&RUN peak enrichment and functional overlap. The CUT&RUN data from Figure 1 was subjected to peak calling using MACS2. BRD4 peaks overlapped with BRG1/ SMARCA4 antibody CUT&RUN peaks (EpiCypher 13-2002, top), as has been demonstrated in the literature (Conrad et al., 2017). Three representative loci show BRD4 peaks in relation to control and BRG1 antibodies (bottom, Integrative Genomics Viewer).

Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.


1. Conrad et al (2017) Mol Cell 12:42

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