SNF2H/SMARCA5 CUTANA CUT&RUN Antibody

CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

Data sheet

Product Description

SNF2H antibody produces CUT&RUN peaks above background that overlap with H3K4me3 (Figure 1), consistent with its known role as the ATP-dependent helicase subunit of the ISWI chromatin remodeler complex (1).


13 2007 Heatmap 300

Figure 1: SNF2H enrichment at annotated transcription start sites (TSSs) in CUT&RUN. CUT&RUN was performed using 500,000 K562 cells with SNF2H (0.1 µg) and control antibodies (0.5 µg; IgG negative control, EpiCypher 13-0042; H3K4me3 positive control, EpiCypher 13-0041). Sequencing reads were aligned to annotated TSSs (+/- 2 kbp) of 18,793 genes. High, medium, and low signal is ranked by intensity (top to bottom) and reflected by red, yellow, and blue colors, respectively. Rows aligned relative to SNF2H antibody.


Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.

References:

1. Santos-Rosa et al (2003) Mol Cell 5:1325-32.

  • Catalog Number
    13-2007-EPC
  • Supplier
    EpiCypher
  • Size
  • Shipping
    Blue Ice
  • Price
  • 599,00 €
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Dr. Sieke Schaepe

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