CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.
CHD3 is an understudied member of the nucleosome remodeling and deacetylase (NuRD) complex. CHD3 antibody produces CUT&RUN peaks above background (Figure 1) within gene promoters, intergenic regions, and introns (Figures 1-2).
Figure 1: CHD3 genome-wide enrichment in CUT&RUN. CUT&RUN was performed using 500,000 K562 cells with 0.5 µg CHD3 antibody. (A) Heatmap of CHD3 peaks (MACS2) relative to IgG and H3K4me3 antibodies (0.5 µg; EpiCypher 13-0042 and 13-0041, respectively). Aligned rows are ranked by CHD3 peak intensity (top to bottom) and colored such that red indicates high enrichment and blue denotes background. (B) The number of CHD3 peaks falling within the indicated genomic features is plotted.
Figure 2: Representative CHD3 CUT&RUN peaks. Two gene loci showing CHD3 peaks at a gene promoter (top) and an intronic region (bottom) are representative of the functional annotation analysis of CHD3 peak localization in CUT&RUN (Figure 1). Images were generated using the Integrative Genomics Viewer (Broad Institute).
Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.