CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

User Manual

Product Description

HA antibody is useful for studies utilizing HA-tagged target proteins. HA Tag antibody produces CUT&RUN peaks (Figure 1) that overlap with GATA3 DNA-binding motifs in breast cancer cells expressing 3xHA-tagged GATA3 transcription factor [1]. 

13 2010 Ha Tag Heatmap 600

Figure 1: HA-tagged protein peaks in CUT&RUN. CUT&RUN was performed using HA antibody (0.5 µg) with 500,000 MDA-MB-231 cells stably expressing 3xHA-tagged GATA3 [1]. Peaks were called using MACS2. (A) Heatmap showing 3xHA-GATA3 peaks relative to IgG and H3K4me3 control antibodies (Cat.No. 13-0042-EPC and 13-0041-EPC, respectively) in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. (B) The number of 3xHA-GATA3 peaks which fall into distinct classes of functionally annotated genomic regions is plotted.

Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.


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Dr. Sieke Schaepe

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