CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

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Product Description

CTCF antibody produces CUT&RUN peaks at promoters (Figure 1A) and to a greater extent within intergenic and intronic regions (Figure 1B). CUT&RUN peaks overlap with known CTCF transcription factor binding motifs. 


13 2014 Ctcf Heatmap 600

Figure 1: CTCF signal enrichment in CUT&RUN. CUT&RUN was performed using 500,000 K562 cells with CTCF antibody (0.125 µg) and IgG control (0.5 µg, Cat.No. 13-0042-EPC). (A) Sequencing reads were aligned to annotated TSSs (+/- 2 kbp) of 18,793 genes. High, medium, and low signal is ranked by intensity (top to bottom; aligned relative to CTCF) and reflected by red, yellow, and blue colors, respectively. (B) CTCF peaks were called using SEACR. The number of CTCF peaks which fall into distinct classes of functionally annotated genomic regions is plotted.

Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.

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