CUTANA DNA Purification Kit

CUTANA CUT&RUN kits, reagents, and assay services map histone PTMs and chromatin-interacting proteins with high resolution, at a fraction of the time and cost of standard ChIP-seq experiments.

User Manual Data sheet

Product Description

  • User-friendly 30 minute-workflow
  • Specifically compatible with low starting material
  • Recovery > 50 bp DNA fragments
  • Delivery of purified, concentrated DNA ready for library preparation and next-generation sequencing

Kit Description

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin is an essential step; one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.

Fig.1. CUT&RUN DNA Fragment Size Distribution Analysis. CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit starting with 500,000 K562 cells. CUT&RUN DNA isolated from IgG Negative Control (13-0042k) and H3K4me3 Positive Control (13-0041k) antibodies was used to prepare paired-end Illumina sequencing libraries. Library DNA was analyzed by Agilent Tapestation®. This analysis confirmed that mononucleosomes were predominantly enriched in CUT&RUN (~300 bp peak represents 150 bp nucleosomes + sequencing adapters).

DNA purified with this kit performes perfectly with the CUTANA ChIC/CUT&RUN Kit.

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  • 280,00 €
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Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16