CUTANA DNA Purification Kit
CUTANA™ CUT&RUN kits, reagents, and assay services map histone PTMs and chromatin-interacting proteins with high resolution, at a fraction of the time and cost of standard ChIP-seq experiments.User Manual Data sheet
- User-friendly 30 minute-workflow
- Specifically compatible with low starting material
- Recovery > 50 bp DNA fragments
- Delivery of purified, concentrated DNA ready for library preparation and next-generation sequencing
Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin is an essential step; one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.