ChIC and CUT&RUN have revolutionized the study of chromatin regulation by enabling targeted release of genomic fragments into solution.

CUTANA CUT&RUN kits, reagents, and assay services map histone PTMs and chromatin-interacting proteins with high resolution, at a fraction of the time and cost of standard ChIP-seq experiments.

Quickstart Guide User Manual Data sheet

Product Description

  • Dramatically reduced background
  • High resolution genomic mapping for histone PTMs and chromatin-associated proteins
  • Small number of cells and only 3-8 million sequencing reads per sample needed
  • Streamlined and cost saving workflow

Kit Description

The CUTANA ChIC/CUT&RUN Kit contains sufficient materials for 48 CUT&RUN samples and is designed for multi-channel sample pipetting in order to realize the increased experimental throughput advantage of CUT&RUN. The kit includes positive (H3K4me3) and negative (IgG) control antibodies. A panel of bead immobilized H3K4 methyl designer nucleosomes (dNucs™) are spiked-in to control samples to directly monitor experimental success and aid troubleshooting (Figure 1). E. coli DNA is added to samples after pAG-MNase cleavage to enable experimental normalization. The kit is compatible with cells and nuclei, including cryopreserved and cross-linked samples. It is recommended to start with 500,000 cells, however comparable data can be generated using as few as 5,000 cells. The inclusion of controls and compatibility with diverse target types, sample inputs, and low cell numbers make the CUTANA ChIC/CUT&RUN Kit ideal for a variety of research applications.

Fig.1. CUTANA H3K4 MetStat Spike-in Controls. DNA-barcoded unmodified and H3K4-methylated dNucs were immobilized to Streptavidin Beads and spiked-in to CUT&RUN samples prior to the addition of either IgG (top) or H3K4me3 (bottom) control antibodies. The shell script available on the product page was used to count instances of each barcoded dNuc in the CUT&RUN sequencing data. The proportion of read counts normalized to on-target (H3K4me3) are shown. The spike-ins confirmed that the control antibody specifically recovered the target dNuc.

DNA for use in this kit can be easily purified with the CUTANA™ DNA Purification Kit.

Storage and Stability

DO NOT FREEZE ENTIRE KIT. Upon receipt, store individual components at room temperature, 4°C and -20°C (see manual for full instructions).

Performance Data

Fig.2. CUT&RUN DNA Fragment Size Distribution Analysis. CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit starting with 500,000 K562 cells. CUT&RUN DNA isolated from IgG Negative Control (13-0042k) and H3K4me3 Positive Control (13-0041k) antibodies was used to prepare paired-end Illumina sequencing libraries. Library DNA was analyzed by Agilent Tapestation®. This analysis confirmed that mononucleosomes were predominantly enriched in CUT&RUN (~300 bp peak represents 150 bp nucleosomes + sequencing adapters).

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Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16