CUTANA pAG-MNase for ChIC/CUT&RUN Workflows

ChIC and CUT&RUN have revolutionized the study of chromatin regulation by enabling targeted release of genomic fragments into solution.

CUTANA CUT&RUN kits, reagents, and assay services map histone PTMs and chromatin-interacting proteins with high resolution, at a fraction of the time and cost of standard ChIP-seq experiments.

User Manual Data sheet

Product Description

Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets & Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag.

Formulation

CUTANA™ pAG-MNase is provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50% glycerol.

Storage and Stability 

Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.


For conversion of the Micrococcal Nuclease generated short DNA fragments into NGS DNA sequencing libraries the Accel NGS 1S DNA Library Kit from our partner Swift Biosiences fits perfectly.

References

Schmid M, Durussel T & LaemmliUK (2004) ChIC and ChEC; genomic mapping of chromatin proteins. Molecular Cell 16:147-157 (doi:10.1016/j.molcel.2004.09.007).

Skene PJ, Henikoff JG & Henikoff S (2018) Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nature Protocols 13:1006-1019 (doi:10.1038/nprot.2018.015).

Skene PJ, Henikoff S (2017) An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. Elife. 2017. 6: p. (PubMed PMID: 28079019) (PMC5310842).

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  • 379,00 €
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Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com