CUTANA pAG-Tn5 for CUT&Tag
CUTANA™ CUT&Tag reagents, kits, and assay services enable rapid, cost-effective epigenomic mapping and deliver high quality data with improved resolution compared to ChIP-seq.User Manual Data sheet
Recombinantly produced in E. coli, CUTANA pAG-Tn5 for CUT&Tag is a fusion of Proteins A and G to Transposase Tn5. This construct is useful in performing Cleavage Under Targets and Tagmentation (CUT&Tag). The active dimer of Transposase Tn5 is charged with Illumina adapters and ready to be used immediately in CUT&Tag. CUTANA pAG-Tn5 does not contain an epitope tag, which makes it compatible with tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, etc.).
This product is sufficient to perform 250 CUT&Tag reactions. Recommended use: 2.5 μL of the supplied enzyme into a 50 μL CUT&Tag reaction (20X dilution). For detailed applications and uses of this product, please see CUT&Tag protocol at: EpiCypher optimized protocols .
NOTE: Since CUT&Tag has lower background and is compatible with fewer cells compared to ChIP-seq, it is not recommended to assess fragment size distribution using agarose gel or capillary electrophoresis (e.g. Agilent Bioanalyzer or TapeStation) prior to library preparation. This analysis is not indicative of the success of a CUT&Tag experiment, and further the amount of DNA recovered may be below the sensitivity of detection for these approaches. To gauge the success of a CUT&Tag experiment, assess DNA yield compared to positive (e.g. H3K4me3, H3K27me3) and negative (IgG) controls, determine fragment size distribution of sequence-ready libraries, and evaluate peak structure and expected genome-wide distribution in NGS data.
(1) Kaya-Okur et. al, Nat. Commun. 2019 (PMID : 31036827)
(2) Henikoff and Henikoff, bioRxiv. 2020 (2020.04.15.043083)