CUTANA High Fidelity 2X PCR Master Mix

User Manual Data sheet

Product Description

CUTANA High Fidelity 2X PCR Master Mix for CUT&Tag is formulated for non-hot start amplification of next generation sequencing (NGS) libraries from CUT&Tag experiments using CUTANA pAG-Tn5. The master mix requires only the addition of primers and the tagmented chromatin to achieve high fidelity amplification of NGS libraries.

Formulation

Q5® High Fidelity thermostable DNA Polymerase (3’→5’ exonuclease activity) fused to Sso7d processivity-enhancing domain in a master mix containing 200 μM dNTPs and 4 mM Mg2+ in proprietary buffer.

Recommended use

After completing pAG-Tn5 chromatin tagmentation, add 25 μL of the supplied enzyme into a ~25 μL CUT&Tag reaction (2X dilution). For detailed instructions regarding non-hot start PCR cycling conditions in CUT&Tag, see CUTANA Direct-to-PCR CUT&Tag protocol: epicypher.com/resources/protocols.

NOTE: Not recommended for use with primers or templates containing uracil.

References

(1) Kaya-Okur et. al, Nat. Commun. 2019 (PMID : 31036827)

(2) Henikoff and Henikoff, bioRxiv. 2020 (2020.04.15.043083)

  • Catalog Number
    15-1018-EPC
  • Supplier
    EpiCypher
  • Size
  • Shipping
    Blue Ice
  • Price
  • 185,00 €
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Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com