Urine microRNA Purification KitUser Manual
|Product Category:||microRNA Purification|
- Isolate all small RNA molecules including miRNA, siRNA, and tRNA
- Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in under 30 minutes
- Versatile urine input volume - Isolate urine microRNA from 0.5 - 1 mL of urine samples
- No phenol or chloroform extractions
- Isolate inhibitor-free RNA suitable for downstream applications
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.
Purified RNA is fully compatible with various downstream applications relating to gene regulation and functional analysis, including RT-PCR, northern blotting and microarray analysis.
Isolation and Detection of microRNA from Urine Samples. Norgen’s Urine microRNA Purification Kit was used to isolate microRNA from 8 different 1.5 mL urine samples. The purified microRNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene. Five microlitres of the isolated RNA was used as the template in the RT step, and 5 ìL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the miR-21 gene in all cases, indicating the high quality of the isolated urine microRNA. The black line in the graph above corresponds to the No Template Control.
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