PARP Antibody (Clone 7A10)

Application

The antibody performs well on Western blot (1:500-1:2000) and Flow Cytometry studies (1:200-1:400).

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability, cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.