Cytochrome c Antibody (Clone 7H8.2C12)

Immunogen

Pigeon cytochrome c

Application

Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.

Background

Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.