Total RNA Purification Micro Kit for small input amounts of cultured animal cells, tissue samples, and microdissected samples including laser-capture microdissection (LCM)

User Manual
Specifications
Product Category: Total RNA incl. microRNA Purification
Sample Type: Low Cell Number Samples

Product Description

  • Isolate total RNA, from large rRNA down to microRNA (miRNA)
  • Isolate high quality total RNA with complete size range from small amounts of cells and tissue, including Laser Captured Microdissections (LCM)
  • RNA can be isolated and detected from as little as a single animal cell (see application note - link below)
  • Small elution volume - Elute total RNA in as little as 20 uL of Elution Buffer
  • No phenol or chloroform extractions
  • Rapid spin column format, protocol completed in 20min
  • Kits for larger input amounts incl. 96-well kit for high throughput applications also available

Norgen's Total RNA Purification Micro Kit provides a rapid method for the isolation and purification of total RNA from small amounts of cultured animal cells, and tissue samples, incl. Laser captured Microdissections. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Purification Technology

Purification is based on spin column chromatography using Norgen's silicon carbide (SiC) resin as the separation matrix.

The process involves first lysing the cells or tissue of interest with the provided Lysis Solution. Binding solution and ethanol are then added to the lysate, and the solution is loaded onto a spin-column. Norgen's resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins and genomic DNA will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed twice with the provided wash buffer in order to remove any remaining impurities, and the purified total RNA is eluted with the elution buffer. The purified RNA is of the highest integrity, and the complete profile of RNA species is represented.

Great Isolation Sensitivity

Norgen’s Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells. Five uL of the eluted RNA was used as the template in a 20 uL RT-qPCR reaction to detect the human S14 transcript. The S14 was detected from as little as a single cell.

High Quality RNA Isolated from Laser-Captured Microdissections (LCM)

Norgen’s Total RNA Purification Micro Kit allows sensitive but high quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen’s Total RNA Purification Kit recovered good amounts of RNA with excellent 260/280 ratio

Applications

  • Price
  • 486,00 €
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