Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit

User Manual
Specifications
Product Category: Circulating Nucleic Acid Purification
Sample Type: Urine

Product Description

  • Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution
  • Versatile urine input ranging from 10 mL - 30 mL
  • No phenol extractions
  • No carrier RNA
  • Concentrate circulating DNA, circulating RNA and exosomal RNA, viral DNA, viral RNA into a flexible elution volume ranging from 50 µL - 100 µL
  • Purify high-quality RNA/RNA in 45 - 50 minutes
  • Compatible with fresh, frozen or preserved urine sample
  • Purified RNA is suitable for a variety of downstream applications, including small RNA sequencing

The Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating including exosomal RNA as well as viral DNA/RNA from fresh, frozen or preserved urine samples from volumes ranging from 10 mL to 30 mL.

Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen’s Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

The purified nucleic acid is of the highest integrity, and can be used in a any downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

  • Catalog Number
    60100-NB
  • Supplier
    Norgen Biotek
  • Size
  • Shipping
    RT
  • Price
  • 264,00 €
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you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com