Endura Chemically Competent Cells (DUOS) (direct replacement for Stbl3 and SURE Chem cells) > 1 x 10e7 cfu/ug

User Manual
Competence: Chemically Competent
Application: Large or Difficult Fragment Cloning, Lentiviral Genome Editing
Organism: E. coli

Product Description

  • Clone unstable sequences (e.g. retroviral elements) with reliable results
  • The cells of choice for propagating lentiviral constructs, e.g. CRISPR Cas9 lentiviral gRNA constructs and libraries, such as the GeCKO (Genome Scale CRISPR Knock Out) pooled lentiviral gRNA libraries.
  • Direct replacement for Invitrogen Stbl3™ and Agilent SURE strain
  • Stable Genotype without resistance marker
  • Excellent value
  • High efficiency: > 1 × 10e7 cfu/ug
  • Your choice of two packaging formats
  • Also available as electrocompetent cells (> 1 × 10e10 cfu/ug)

Lucigen’s Endura Competent Cells (Genotype: recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–) are a commonly used strain for cloning, maintaining and stably propagating sequences that suffer unwanted recombination events in other strains, e.g. inverted repeats or other sequences prone to recombination (commonly found for example in retroviral genes).

Chemical or electrocompetent, whichever method of transformation you prefer, Lucigen's Endura cells give you higher efficiency at better prices.

Order Information:

Endura™ Competent Cells include Control DNA and Recovery Medium, and are available packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Custom Configurations

Please contact us for more information related to custom competent cell services or to receive a bulk quotation.

  • Price
  • 285,00 €
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you need any help?

Please contact:

Maria Weber

Tel. +49 (0) 6221 71415 16 info@biocat.com