Arima-HiChIP

Discover active gene regulation interactions. The easy-to-use and reproducible Arima-HiChIP approach streamlines the HiChIP protocol via a 2-day workflow and is optimized for efficient capture of chromatin looping (3D) and ChIP enrichment (1D) data. It facilitates broad and easy access to the powerful HiChIP technology.

  • Discovery of active gene regulatory interactions at high resolution
  • Compatible with a range of commercially available library prep kits
  • Demonstrated differential loop calling between sample types
  • Robust across cell types and protein targets
  • Reduced sequencing costs
  • 2 day workflow with simple protocol for first time-users
Application Note

Product Description

The Arima-HiChIP protocol is a reproducible and simplified protocol for identifying long-range genomic contacts mediated by proteins. HiChIP combines Hi-C and chromatin immunoprecipitation with antibodies against a protein of interest to enrich for interactions mediated by this protein. As a targeted approach HiChIP has the advantage of requiring less sequencing compared to Hi-C. Whereas Hi-C can potentially detect all types of chromatin interactions, Arima HiChIP targeting H3K27ac or H3K4me, using Arima validated antibodies, is specific for interactions linking active regulatory domains (see application note).

Arima-HiChIP Library Preparation

Arima-HiChIP is a 2-day protocol that results in proximally-ligated DNA that was associated with an immunoprecipitated protein of interest. This enriched DNA is then prepared as an Arima-HiChIP library in 1-day using a pre-validated commercially available library prep kit, namely the Accel-NGS 2S Plus DNA Library Kit from Swift Biosciences. After library prep, the resulting Arima-HiChIP libraries are sequenced in paired-end mode via Illumina next generation sequencers.

Arima Hichip Workflow

Fig.1. Arima-HiChIP Experimental Workflow

Applications

  • Discovery of active gene regulatory interactions
  • Mapping of CTCF-mediated genome folding using H3K27ac and H3K4me3
  • Studies of promoter-enhancer communication

Introduction to Arima-HiChip

Product Citations

  • Catalog Number
    A101020-ARI
  • Supplier
    Arima
  • Size
  • Shipping
    Blue Ice
  • Price
  • 3.320,00 €
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Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 714 15 16 info@biocat.com