293AAV Cell LineData sheet
|Cell Category:||Packaging Cell Lines|
- Larger cell surface area resulting higher transfection and better yield of AAV
- Flattened morphology
- Firm attachment to culture plate, ideal for large scale culture and AAV production
Adeno-associated virus (AAV) belongs to the family of Parvoviridae, a group of viruses among the smallest of single-stranded and non-enveloped DNA viruses. There are nine different AAV serotypes reported to date. AAV can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for long-term gene transfer. Recombinant AAV-2 is the most common serotype used in gene delivery, and can be produced at high titers with helper virus or helper-free system in 293 cells.
The 293AAV Cell Line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein.
293AAV is derived from the parental 293 cell line, through cloning and multiple rounds of testing, 293AAV is specifically selected for a high level of AAV production in a helper-free system.
Genetically modified organism (GVO), see General Terms