BRCA1 and BRCA2 Accel-Amplicon® Panel
Targeted Sequencing for Breast Cancer GenesUser Manual Brochure
The Accel-Amplicon BRCA1 and BRCA2 Panel offers comprehensive coverage of the entire coding sequence of BRCA1 and BRCA2 genes. Utilizing 246 amplicons with average size of 148 bp, this panel generates targeted libraries compatible with Illumina® and Ion Torrent sequencing platforms.
The unique design of the Accel-Amplicon BRCA1 and BRCA2 Panel enables compatibility with FFPE and cfDNA samples. This product is a complete kit that includes all components necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters.
- Single-tube assay
- Ready-to-sequence libraries in 2 hours
- Inputs as low as 10 ng
- Average amplicon size of 148 bp for compatibility with FFPE and cfDNA
- Highly sensitive detection of rare variants down to 1% allele frequency.
- Complete library generation in a single kit
- Streamlined analysis with bioinformatics resources such as Primerclip and Genialis.
- Compatible with all Illumina platforms
Extensive coverage of tumor suppressor genes implicated in breast & ovarian cancer
The Accel-Amplicon Plus BRCA 1 & BRCA 2 Panel is a fast, easy assay for variant discovery and screening. It offers comprehensive coverage of the entire coding sequence and flanking exon/intron boundaries of the BRCA1 and BRCA2 genes. It’s designed for compatibility with difficult and low input samples, such as FFPE and cell-free DNA for liquid biopsy analysis. But that’s just the beginning.
Comprehensive: Covers over 1,400 COSMIC and 13,000 ClinVar mutations and generates targeted libraries compatible with Illumina® sequencing platforms.
Powerful: Simultaneous detection of single nucleotide variants (SNV), copy number variants (CNV) and small insertion and deletions (indels), all within a fast and easy single-tube assay workflow.
The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.
*As quantified by qPCR. Qubit® represents amplifiable DNA content more accurately than NanoDrop®, however it is still not as accurate as the qPCR assay. For sample types with more consistent high quality DNA including whole blood, fresh frozen samples, and cultured cells, quantification by Qubit is a reliable indicator of amplifiable content.
Achieve Robust Sequencing Performance Over a Wide Range of Samples
An array of control DNA samples (10 ng input for each) was used to generate libraries with the Accel-Amplicon Plus BRCA1 and BRCA2 Panel. The samples include male and female Coriell repository DNAs of different ethnicities, Horizon HD701 quantitative multiplex reference standard, the Acrometrix™ Oncology Hotspot Control, gDNA and cfDNA extracted from human blood, circulating cell-free DNA, and three formalin-compromised samples, including Horizon HD200 FFPE. Libraries were sequenced on an Illumina MiniSeq instrument and the on target aligned reads and coverage uniformity percentages were plotted.
Sequencing data files (FASTQ, BED, BAM, and VCF) for this amplicon panel are available on request.