Comprehensive TP53 Accel-Amplicon® Panel
Complete coverage of the tumor suppressor gene involved in cell cycle, DNA repair and programmed cell deathUser Manual Brochure
The Accel-Amplicon Plus Comprehensive TP53 Gene Panel offers complete coverage of the entire coding sequence and flanking exon/intron boundaries of the TP53 gene. It’s fully compatible with difficult and low input samples, such as FFPE and cell-free DNA for liquid biopsy analysis. But that’s just the beginning.
The panel is modular, allowing you to add on focused content to achieve your most optimal design. Either combine it with our pre-validated content or add your own targets. Swift will work alongside you to design your assay and then optimize and wet-bench validate its performance before shipping it to you.
Comprehensive: Covers 1,000 COSMIC and 700 ClinVar mutations and generates targeted libraries compatible with Illumina® sequencing platforms
Powerful: Simultaneous detection of single nucleotide variants (SNV) and small insertions and deletions (indels), all within a fast and easy single-tube assay workflow.
Flexible: Layer on pre-validated content from other Accel-Amplicon Plus Panels (e.g. BRCA1 and BRCA2 Panel, Lynch Syndrome) or your own targets. The possibilities are endless.
- Robust performance on cell-free DNA and FFPE samples
- Inputs as low as 10 ng
- Highly sensitive detection of rare variants down to 1% allele frequency
- Easy, single-tube assay with sequence ready libraries in under 2 hours
- Streamlined analysis with bioinformatics resources including Primerclip and Genialis
- Compatible with all Illumina sequencers
The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.
Achieve Robust Sequencing Performance Over a Wide Range of Samples
An array of control DNA samples (10 ng input for each) was used to generate libraries with the Accel-Amplicon Plus Comprehensive TP53 Panel. The samples include male and female Coriell repository DNAs of different ethnicities, Horizon HD701 quantitative multiplex reference standard, the Acrometrix™ Oncology Hotspot Control, high molecular weight genomic DNA, circulating cell-free DNA, and three formalin-compromised samples, including Horizon HD200 FFPE equivalent of HD701. Libraries were sequenced on an Illumina MiniSeq instrument and the on target aligned reads and coverage uniformity percentages were plotted.